QuEChERS Sample Preparation Kits | Thermo Fisher Scientific - US

 
 
 
QuEChERS Sample Preparation—Now Easier, More Convenient and Affordable

QuEChERS sample preparation—now easier, more convenient, and affordable

QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) is a sample preparation method that was initially developed in for analyzing veterinary drugs in food. This innovative method was also successfully tested for pesticide residue analysis in plant materials, demonstrating its versatility and effectiveness. The detailed method was published in the journal AOAC International in . Since then, the QuEChERS method has evolved into a widely adopted sample preparation technique used for a variety of analytes across an expanding number of sample types before analysis by gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS).

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Thermo Scientific QuEChERS kits are designed to streamline your workflow and minimize the potential for error by providing pre-packaged, ready-weighed salts, sorbents, and buffers. These kits are tailored to your specific analytes of interest, sample matrix, and preferred QuEChERS method, ensuring optimal performance and convenience. The available methods include:
✓Original Method: The foundational method that started it all, providing a robust and reliable approach to sample preparation.
✓AOAC .01 Method: A refined version of the original method, offering enhanced performance for specific analytes and matrices.
✓EN Method: A European standard method that provides a comprehensive approach to pesticide residue analysis.
✓NY/T - Method: A method tailored to specific regulatory requirements and analytical needs.

Thermo Scientific offers complete packages that include both extraction and clean-up kits, as well as extraction-only and clean-up-only kits to suit your laboratory’s specific sample preparation needs. These pre-packaged kits save time and money, simplify QuEChERS procedures, and ensure high-quality results. By using these kits, you can achieve reproducible results and excellent recoveries for a wide variety of analytes, making your analytical testing more efficient and reliable.

 
 
 
Thermo Scientific QuEChERS standard step-by-step workflow

As shown in the workflow diagram below, the QuEChERS method comprises four basic steps:

1. Homogenize Sample (Fruit or Vegetable): Begin by thoroughly homogenizing the sample, such as a fruit or vegetable, to ensure a uniform consistency. This step is crucial for accurate and representative analysis, as it ensures that the analytes are evenly distributed throughout the sample.
2. Extract Analytes of Interest: Extract the analytes of interest from the homogenized sample using specific extraction salts. These analytes can include pesticide residues, veterinary drugs, and/or mycotoxins. The extraction process involves adding the salts to the sample, which facilitates the release of the target compounds into the solution.
3. Clean-Up the Resulting Supernatant: Clean up the resulting supernatant using dispersive solid phase extraction (dSPE). This step involves adding sorbents to the supernatant to remove unwanted matrix components that could interfere with the analysis. The dSPE process helps to purify the extract, ensuring that the final sample is free from contaminants and ready for accurate analysis.
4. Analyze by GC-MS or LC-MS: Finally, analyze the cleaned-up extract using gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS). These powerful analytical techniques allow for the precise identification and quantification of the target analytes. The choice between GC-MS and LC-MS depends on the specific properties of the analytes and the requirements of the analysis.

By following these four basic steps, the QuEChERS method provides a streamlined and efficient approach to sample preparation, enabling reliable and reproducible results for a wide range of analytes.

 
 
 
QuEChERS Range of Products

A Range of QuEChERS extraction kits for original method

The original QuEChERS method (Anastassiades et al., ) was developed by Steven J. Lehotay and Michelangelo Anastassiades in for the determination of pesticide residues in fruits and vegetables. This pioneering method was specifically designed to analyze non-base-sensitive compounds and employs a sodium chloride buffer to reduce polar interferences and enhance the efficiency of the extraction process.

Buffering is a critical component of the QuEChERS procedure because it allows for the accurate analysis of pesticides and other residues that display pH dependence in their recoveries, depending on the sample matrix. The buffering process helps to stabilize the pH levels, ensuring that the target analytes are effectively extracted and accurately quantified.

To support the original QuEChERS method, three different extraction kits are available

Original method extraction kits

Part numberExtraction saltsAlso includesStarting sampleUnit sizePurchaseS1-10-ORIG-CH-KIT

• 4 g MgSO4
• 1 g NaCl
• In pouches

• 50 mL tubes
• Ceramic homogenizers

10 g sample50/PK

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S1-10-ORIG-KIT

• 4 g MgSO4
• 1 g NaCl
• In pouches

50 mL tubes10 g sample50/PK

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S1-10-ORIG-POT

• 4 g MgSO4
• 1 g NaCl
• In pouches

̶10 g sample50/PK

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S1-15-ORIG-CH-KIT

• 6 g MgSO4
• 1.5 g NaCl
• In pouches

• 50 mL tubes
• Ceramic homogenizers

15 g sample50/PK

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S1-15-ORIG-KIT

• 6 g MgSO4
• 1.5 g NaCl
• In pouches

50 mL tubes15 g sample50/PK

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S1-15-ORIG-POT

• 6 g MgSO4
• 1.5 g NaCl
• In pouches

-15 g sample50/PK

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Chemicals used in the original method

Acetonitrile:Used to perform single-phase extraction (SPE) on a sample. Acetonitrile is a polar organic solvent that effectively extracts a wide range of analytes, including pesticides, veterinary drugs, and mycotoxins, from the sample matrix. Its miscibility with water makes it ideal for extracting both polar and non-polar compounds, ensuring comprehensive analyte recovery.Anhydrous MgSO4:Induces phase separation between the water content in the sample and the acetonitrile layer during the liquid-liquid partitioning step. This separation is crucial for isolating the analytes in the organic phase.
Removes remaining water from the sample during the dispersive solid phase extraction (dSPE) step. Anhydrous magnesium sulfate acts as a drying agent, ensuring that the final extract is free from excess moisture, which could interfere with subsequent analytical steps.NaCl:Induces phase separation between the water content in the sample and the acetonitrile layer during the liquid-liquid partitioning step. Sodium chloride helps to increase the ionic strength of the aqueous phase, promoting the partitioning of analytes into the acetonitrile layer.
Removes residual water from the sample, enhancing the efficiency of the extraction process.
Reduces polar interference and enhances extraction by minimizing the solubility of polar compounds in the aqueous phase, thereby improving the recovery of target analytes in the organic phase.PSA (Primary Secondary Amine):Used to remove polar matrix components, citric co-extractives, and free fatty acids from the sample during the dispersive solid phase extraction (dSPE) step. PSA is a sorbent that selectively binds to and removes these interfering substances, ensuring that the final extract is clean and suitable for accurate analysis by GC-MS or LC-MS.

QuEChERS extraction and clean-up kits for AOAC .01 method

The AOAC .01 QuEChERS Method, developed by Steven J. Lehotay and his co-workers, refines the original method by incorporating higher concentrations of acetate buffering for enhanced buffering strength. Buffering is essential in the QuEChERS procedure as it stabilizes pH during extraction and clean-up steps, ensuring accurate analysis of pH-dependent pesticides and other residues.

The method starts with homogenizing the sample, such as fruits or vegetables, to achieve uniform consistency. Extraction salts, including magnesium sulfate and sodium acetate, are then added to release analytes into the acetonitrile solvent. The mixture undergoes liquid-liquid partitioning, where anhydrous magnesium sulfate and sodium chloride induce phase separation and remove residual water.

The supernatant is cleaned using dispersive solid phase extraction (dSPE) with sorbents like primary secondary amine (PSA) to remove polar matrix components. The purified extract is then analyzed by GC-MS or LC-MS for precise identification and quantification. Thermo Scientific provides pre-packaged kits to support this method, streamlining workflows and ensuring high-quality, reproducible results.

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AOAC .01 method extraction kits

Part numberExtraction saltsAlso includesStarting sampleUnit sizePurchaseS1-15-AOAC-CH-KIT

• 6 g MgSO4
• 1.5 g NaOAc
  
• In pouches

• 50 mL tubes
• Ceramic homogenizers

• 15 g sample

• 50/PK

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S1-15-AOAC-KIT

• 6 g MgSO4
• 1.5 g NaOAc
  
• In pouches

• 50 mL tubes

• 15 g sample

• 50/PK

Buy now

S1-15-AOAC-POT

• 6 g MgSO4
• 1.5 g NaOAc
  
• In pouches

̶

• 15 g sample

• 50/PK

Buy now

AOAC .01 method clean-up kits

Sample typePrefilled 2 mL tubes
(100/PK)Prefilled 15 mL tubes
(50/PK) 
Clean-upGeneral fruits and vegetablesS2-2-GFV-AOAC-KIT

• 50 mg PSA
• 150 mg Anhydrous MgSO4

S2-15-GFV-AOAC-KIT

• 400 mg PSA
• mg Anhydrous MgSO4

Pigmented fruits and vegetablesS2-2-P-AOAC-KIT

• 50 mg PSA
• 50 mg GCB
• 150 mg MgSO4

S2-15-P-AOAC-KIT

• 400 mg PSA
• 400 mg C18
• mg Anhydrous MgSO4

Fruits and vegetables with fats and waxesS2-2-FW-AOAC-KIT

• 50 mg PSA
• 50 mg C18
• 150 mg Anhydrous MgSO4

S2-15-FW-AOAC-KIT

• 400 mg PSA
• 400 mg C18
• mg Anhydrous MgSO4

Fruits and vegetables with pigments and fatsS2-2-PF-AOAC-KIT

• 50 mg PSA
• 50 mg C18
• 50 mg GCB
• 150 mg Anhydrous MgSO4

S2-15-PF-AOAC-KIT

• 400 mg PSA
• 400 mg C18
• 400 mg GCB
• mg Anhydrous MgSO4

All food typesS2-2-ALL-AOAC-KIT

• 50 mg PSA
• 50 mg C18
• 7.5 mg GCB
• 150 mg MgSO4

S2-15-ALL-AOAC-KIT

• 400 mg PSA
• 400 mg C18
• 45 mg GCB
• mg MgSO4

Other foodsS2-2-OTH-AOAC-KIT

• 25 mg C18
• 150 mg MgSO4

S2-15-OTH-AOAC-KIT

• 150 mg C18
• 900 mg MgSO4

Chemicals used in the AOAC .01 method

Acetonitrile:Used to perform single-phase extraction on a sample. It effectively extracts a wide range of analytes, including pesticides, veterinary drugs, and mycotoxins. Its miscibility with water allows for efficient extraction of both polar and non-polar compounds.Anhydrous MgSO4:Induces phase separation between the water content in the sample and the acetonitrile layer during the liquid-liquid partitioning step. It removes remaining water from the sample during the dispersive solid phase extraction (dSPE) step, acting as a drying agent to ensure the final extract is free from excess moisture.NaOAc (sodium acetate):Buffers the sample to stabilize pH. This buffering is essential for maintaining consistent pH levels during the extraction process, ensuring accurate recovery of pH-dependent analytes.PSA (primary secondary amine):

Used during the dSPE step to remove polar matrix components, citric co-extractives, and free fatty acids from the sample, ensuring that the final extract is clean and suitable for accurate analysis.

C18:Removes fats, sterols, and other non-polar interferences from the sample, reducing the presence of non-polar contaminants that could interfere with the analysis.GCB (graphitized carbon black):Removes pigments from the sample. It is not recommended for use with planar pesticides as it may also remove these target analytes. GCB is particularly effective in cleaning up samples with high pigment content, ensuring a clearer extract for analysis.

QuEChERS extraction and clean-up kits for EN method

The extraction and clean-up products here support the EN method. Widely used in Europe, the EN method was developed by Michelangelo Anastassiades and co-workers, who modified the original method to use citrate buffering at a relatively low buffering capacity. This variation employs a sodium chloride buffer to minimize polar interferences, along with sodium citrate dihydrate and disodium citrate sesquihydrate buffers. Buffering is a crucial part of the QuEChERS procedure because it stabilizes the pH, allowing for accurate analysis of pesticides and other residues that exhibit pH dependence in their recoveries, depending on the sample matrix. This ensures consistent and reliable results across various sample types.

EN method extraction kits

Part numberExtraction saltsAlso includesStarting sampleUnit sizePurchaseS1-10-EN-CH-KIT

• 4 g MgSO4
• 1 g NaCl
• 0.5 g disodium hydrogencitrate sesquihydrate
• 1 g trisodium citrate dihydrate
• In pouches

• 50 mL tubes
• Ceramic homogenizers

10 g50/PK

Buy now

S1-10-EN-KIT

• 4 g MgSO4
• 1 g NaCl
• 0.5 g disodium hydrogencitrate sesquihydrate
• 1 g trisodium citrate dihydrate
• In pouches

50 mL tubes10 g50/PK

Buy now

S1-10-EN-POT

• 4 g MgSO4
• 1 g NaCl
• 0.5 g disodium hydrogencitrate sesquihydrate
• 1 g trisodium citrate dihydrate
• In pouches

-10 g50/PK

Buy now

EN method clean-up kits

Sample typePrefilled 2 mL tubes
(100/PK)Prefilled 15 mL tubes
(50/PK)

Chemicals used in the EN method

Acetonitrile:Used to perform single-phase extraction on a sample. It serves as a polar organic solvent that effectively extracts a wide range of analytes, including pesticides and residues, from the sample matrix. Its miscibility with water allows for efficient extraction of both polar and non-polar compounds.  Anhydrous MgSO4:Induces phase separation between the water content in the sample and the acetonitrile layer during the liquid-liquid partitioning step. Additionally, anhydrous magnesium sulfate removes remaining water from the sample during the dispersive solid phase extraction (dSPE) step, acting as a drying agent to ensure the final extract is free from excess moisture.NaCl:Induces phase separation between the water content in the sample and the acetonitrile layer during the liquid-liquid partitioning step and helps to remove residual water from the sample. This step enhances the efficiency of the extraction process by reducing polar interferencePSA (Primary Secondary Amine):Used during the dSPE step to remove polar matrix components, citric co-extractives, and free fatty acids from the sample, ensuring that the final extract is clean and suitable for accurate analysis.C18:Used to remove fats, sterols, and other non-polar interferences from the sample, reducing the presence of non-polar contaminants that could interfere with the analysis.GCB (Graphitized Carbon Black):Used to remove pigments from the sample. However, it is not recommended for use with planar pesticides as it may also remove these target analytes. GCB is particularly effective in cleaning up samples with high pigment content, ensuring a clearer extract for analysis.

QuEChERS clean-up kit for NY/T - method

The QuEChERS clean-up kit for the NY/T - method is recommended by the China Ministry of Agriculture. This method is designed for the sample preparation of 51 specific pesticide residues in fruits and vegetables, ensuring accurate and efficient analysis by GC-MS. It streamlines the process for reliable results.

NY/T - method clean-up kit

SamplePrefilled 15 mL tubes
(50/PK)PurchaseFruits and vegetablesS2-15-GFV-NYT-KIT

• 100 mg PSA
• 100 mg C18
• 300 mg Anhydrous MgSO4

Buy now

Chemicals used in the NY/T - method

Anhydrous MgSO4:Induces phase separation between the water content in the sample and the acetonitrile layer during the liquid-liquid partitioning step. It also removes remaining water from the sample during dispersive solid phase extraction (dSPE).  PSA (Primary Secondary Amine):Used during the dSPE step to remove polar matrix components, citric co-extractives, and free fatty acids from the sample, ensuring a clean extract for accurate analysis.C18:Employed to remove fats, sterols, and other non-polar interferences from the sample, reducing the presence of contaminants that could interfere with the analytical results.

 
 
 
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VetDrugs Explorer Collection

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TriPlus RSH SMART Autosampler

Automated, high-throughput workflows for GC and LC automates QuEChERS clean-up of extracts

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Related consumables

Part numberProduct description

Vials & caps

6ASV9-1P2mL clear screw glass vial (9mm short thread) with write-on patch6ESV9-1PP1.5mL clear screw PP vial (9mm short thread)6ASV9-2P2mL amber screw glass vial (9mm short thread) with write-on patch6ASC9ST1RRed screw cap (9mm) (PP: white silicone/red PTFE septa: 1.0mm thickness) with AVCS technology6ASC9ST1Blue screw cap (9 mm) (PP: white silicone/blue PTFE septa: 1.0 mm thickness) with AVCS technologyPart numberProduct description

Solvents

A956-1Thermo Scientific UHPLC-MS grade acetonitrile suitable for LC and GCA458-1Thermo Scientific UHPLC-MS grade methanol suitable for LC and GCW8-1Thermo Scientific UHPLC-MS grade water suitable for LC and GCA117Fisher Chemical Optima LC-MS grade formic acid suitable for LC and GCA115-50Fisher Chemical Optima LC-MS grade ammonium formate suitable for LC and GCTThermo Scientific Chromplete acetonitrile suitable for HPLC and GCDOptima Methylene Chloride for suitable HPLC and GC-MSHOptima 95% n-Hexane suitable for HPLC and GC-MSSSodium sulfate anhydrous suitable for LC and GCPart numberProduct description

HPLC columns

-Thermo Scientific Accucore aQ (100 × 2.1 mm, 2.6 μm)Thermo Scientific Acclaim Trinity P1 (anionic pesticides) (100 × 3.0 mm, 3.0 µm)-Thermo Scientific Accucore RP-MS (100 × 2.1 mm, 2.6 μm)-Thermo Scientific Accucore aQ (100 × 2.1 mm, 2.6 μm)Part numberProduct description

GC consumables

453TThermo Scientific LinerGold: Siltek six baffle PTV liner453AUISSL splitless liner, single taper, deactivated-Thermo Scientific TraceGOLD TG-5SIL MS (30 m x 0.25 mm i.d. x 0.25 μm)

 
 
 
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